基于SELDI-TOF-MS技术的患亚临床低血钙症奶牛血浆蛋白质组谱分析

doi:10.11843/j.issn.0366-6964.2014.11.022

Abstract

Abstract:

The aim of this study was to determine the differences between the post-parturition plasma proteomic profiles of healthy cows and those with subclinical hypocalcemia (SH).Plasma samples were obtained from 32 dairy cows with SH (group T) and 59 dairy cows without SH (group C).Plasma proteins were tested by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).The peaks that differ in the two groups were analyzed using the Wilcoxon sum-rank test.The differential peptide peaks were identified using the Swissprot protein database，which were performed by decision tree analysis.Seven peptide peaks were identified in the group T that differed significantly from those of the group C，which represented 6 unique proteins.Further，neurosecretory protein VGF frag and amyloid β A4 protein could be used for discriminate the cows with SH from the health after decision tree analysis.The SELDI-TOF-MS technology can effectively identify differences in serum protein expression patterns in cows with SH，which may become biomarkers for disease diagnosis.Therefore，this study has important theoretical value to reveal new mechanism of subclinical hypocalcemia and its effect on bio-function of dairy cows.

CLC Number:

S856.5

WANG Peng-xian，SHU Shi，WANG Bo，XU Chu-chu，LIU Jian-nan，XIA Cheng，WU Ling，WANG Zhe. SELDI-TOF-MS Analysis of the Post-parturition Plasma Proteomic Profile of Dairy Cows with Subclinical Hypocalcemia[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1895-1903.

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SELDI-TOF-MS Analysis of the Post-parturition Plasma Proteomic Profile of Dairy Cows with Subclinical Hypocalcemia

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